PE Anti-Human CD163 (GHI/61)
CD163 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human and More (1)
Applications
FC
Conjugate
PE Fluorescent Dye
CloneNo.
GHI/61
验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
Planning an IHC experiment? We recommend our IHCeasy CD163 Ready-To-Use IHC Kit. CD163 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| FC | See 2 publications below |
产品信息
PE-65169 targets CD163 in FC applications and shows reactivity with Human samples.
| Tested Applications | FC Application Description |
| Cited Applications | FC |
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Immunogen | Glycoprotein preparation from hairy cell leukemia spleen 种属同源性预测 |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD163 molecule |
| Synonyms | CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163 |
| Calculated Molecular Weight | 1156 aa, 125 kDa |
| GenBank Accession Number | BC051281 |
| Gene Symbol | CD163 |
| Gene ID (NCBI) | 9332 |
| ENSEMBL Gene ID | ENSG00000177575 |
| RRID | AB_2883945 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
| Form | Liquid |
| Purification Method | The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| UNIPROT ID | Q86VB7 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for PE CD163 antibody PE-65169 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Discov Suppression of the hyaluronic acid pathway induces M1 macrophages polarization via STAT1 in glioblastoma. | ||
Cell Mol Biol Lett LINC00330/CCL2 axis-mediated ESCC TAM reprogramming affects tumor progression |
