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CoraLite® Plus 555 Anti-Human CD163 (GHI/61)

CD163 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

GHI/61

Cat no : CL555-65169

Print datasheet

Synonyms

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

验证数据展示

  • FC experiment of human PBMCs using CL555-65169

    FC experiment of human PBMCs using CL555-65169

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD163 (CL555-65169, Clone:GHI/61) (red) or unstained. Cells were not fixed. Monocytes and granulocytes were gated.

  • FC experiment of human PBMCs using CL555-65169

    FC experiment of human PBMCs using CL555-65169

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD163 (CL555-65169, Clone:GHI/61) or unstained. Cells were not fixed. Monocytes and granulocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD163 Ready-To-Use IHC Kit. CD163 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65169-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-65169 targets CD163 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Glycoprotein preparation from hairy cell leukemia spleen 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD163 molecule
Synonyms CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163
Calculated Molecular Weight 1156 aa, 125 kDa
GenBank Accession NumberBC051281
Gene Symbol CD163
Gene ID (NCBI) 9332
ENSEMBL Gene IDENSG00000177575
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
UNIPROT IDQ86VB7
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 CD163 antibody CL555-65169Download protocol
Standard Protocols
Click here to view our Standard Protocols