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验证数据展示

  • FC experiment of human PBMCs using CL555-65169

    FC experiment of human PBMCs using CL555-65169

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD163 (CL555-65169, Clone:GHI/61) (red) or unstained. Cells were not fixed. Monocytes and granulocytes were gated.

  • FC experiment of human PBMCs using CL555-65169

    FC experiment of human PBMCs using CL555-65169

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD163 (CL555-65169, Clone:GHI/61) or unstained. Cells were not fixed. Monocytes and granulocytes were gated.

CoraLite® Plus 555 Anti-Human CD163 (GHI/61)

CD163 Monoclonal Antibody for FC
Cat No. CL555-65169

产品说明书

CloneNo. GHI/61

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human

应用

FC

别名

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 555
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD163 Ready-To-Use IHC Kit. CD163 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65169-1-Ig

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-65169 targets CD163 in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原 Glycoprotein preparation from hairy cell leukemia spleen 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD163 molecule
别名 CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163
计算分子量 1156 aa, 125 kDa
GenBank蛋白编号BC051281
基因名称 CD163
Gene ID (NCBI) 9332
ENSEMBL Gene IDENSG00000177575
RRIDAB_3084576
偶联类型 CoraLite® Plus 555 Fluorescent Dye
最大激发/发射波长554 nm / 570 nm
形式 Liquid
纯化方式Purified by protein-A affinity chromatography
UNIPROT IDQ86VB7
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 CD163 antibody CL555-65169Download protocol
Standard Protocols
Click here to view our Standard Protocols