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FITC Anti-Mouse CD18 (M18/2)

CD18 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

FITC Fluorescent Dye

CloneNo.

M18/2

Cat no : FITC-65178

Print datasheet

Synonyms

2E6, AI528527, Cd18, integrin beta 2, Itgb2, LAD, LCAMB, Lfa1, MF17

验证数据展示

  • FC experiment of mouse splenocytes using FITC-65178

    FC experiment of mouse splenocytes using FITC-65178

    1X10^6 mouse splenocytes were surface stained with 0.5 ug FITC Anti-Mouse CD18 (FITC-65178, Clone: M18/2) (red), or 0.5 ug Control Antibody. Cells were not fixed.

  • FC experiment of mouse splenocytes using FITC-65178

    FC experiment of mouse splenocytes using FITC-65178

    1X10^6 mouse splenocytes were surface stained with 0.5 ug FITC Anti-Mouse CD18 (FITC-65178, Clone: M18/2) (red), or 0.5 ug Control Antibody. Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65178 targets CD18 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen Mouse CTL glycoprotein 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name integrin beta 2
Synonyms 2E6, AI528527, Cd18, integrin beta 2, Itgb2, LAD, LCAMB, Lfa1, MF17
GenBank Accession NumberBC145644
Gene Symbol CD18
Gene ID (NCBI) 16414
RRIDAB_2883816
Conjugate FITC Fluorescent Dye
Excitation/Emission Maxima Wavelengths494 nm / 520 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
UNIPROT IDP11835
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

实验方案

Product Specific Protocols
FC protocol for FITC CD18 antibody FITC-65178Download protocol
Standard Protocols
Click here to view our Standard Protocols