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验证数据展示

  • FC experiment of human PBMCs using Biotin-98134

    FC experiment of human PBMCs using Biotin-98134

    1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD2 Rabbit Recombinant Antibody (Biotin-98134, Clone: 241222E2) and CoraLite®488-conjugated streptavidin, or unstained. Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using Biotin-98134

    FC experiment of human PBMCs using Biotin-98134

    1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD2 Rabbit Recombinant Antibody (Biotin-98134, Clone: 241222E2) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

Biotin Anti-Human CD2 Rabbit Recombinant Antibody

CD2 Recombinant Antibody for FC
Cat No. Biotin-98134

Print datasheet

CloneNo. 241222E2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor, CD2 molecule

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Biotin
Size: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-98134 targets CD2 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD2 molecule
Synonyms LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor, CD2 molecule
Calculated Molecular Weight 351 aa, 39 kDa
GenBank Accession NumberBC033583
Gene Symbol CD2
Gene ID (NCBI) 914
ENSEMBL Gene IDENSG00000116824
RRIDAB_3672380
Conjugate Biotin
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06729
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

实验方案

Product Specific Protocols
FC protocol for Biotin CD2 antibody Biotin-98134Download protocol
Standard Protocols
Click here to view our Standard Protocols