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Anti-Human CD200 Rabbit Recombinant Antibody, PBS Only

CD200 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

230268E6

Cat no : 98007-1-PBS

Print datasheet

Synonyms

OX 2, MRC, MOX2, MOX1, CD200 molecule

验证数据展示

  • FC experiment of human PBMCs using 98007-1-RR (same clone as 98007-1-PBS)

    FC experiment of human PBMCs using 98007-1-RR (same clone as 98007-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 (98007-1-RR, Clone:230268E6) or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then co-stained with APC Anti-Human CD19 (HIB19) (APC-65110, Clone: HIB19). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98007-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98007-1-PBS

    FC experiment of human PBMCs using 98007-1-PBS

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 (98007-1-PBS, Clone:230268E6) or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then co-stained with APC Anti-Human CD19 (HIB19) (APC-65110, Clone: HIB19). Cells were not fixed. Lymphocytes were gated.

  • Affinity and Kinetic Characterization of 98007-1-RR

    Affinity and Kinetic Characterization of 98007-1-RR

    Surface Plasmon Resonance (SPR) kinetic assays of 98007-1-RR against Human CD200 were performed. The affinity constant is 2.687 nM.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98007-1-PBS targets CD200 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD200 molecule
Synonyms OX 2, MRC, MOX2, MOX1, CD200 molecule
Calculated Molecular Weight 269 aa, 30 kDa
GenBank Accession NumberBC022522
Gene Symbol CD200
Gene ID (NCBI) 4345
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP41217
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).

实验方案

Product Specific Protocols
FC protocol for CD200 antibody 98007-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols