CoraLite® Plus 750-conjugated CD22 Recombinant antibody

CD22 Uni-rAbTM Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230550C11

Cat no : CL750-83303

Print datasheet

Synonyms

CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22



经过测试的应用

Positive WB detected inRaji cells, Daudi cells, Ramos cells
Planning an IHC experiment? We recommend our IHCeasy CD22 Ready-To-Use IHC Kit. CD22 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83303-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL750-83303 is CD22 Fusion Protein expressed in E. coli.

Tested Applications WB Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD22 molecule
Synonyms CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22
Calculated Molecular Weight95kd
Observed Molecular Weight130 kDa
GenBank Accession NumberNM_001771.4
Gene Symbol CD22
Gene ID (NCBI) 933
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20273
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 CD22 antibody CL750-83303Download protocol
Standard Protocols
Click here to view our Standard Protocols