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CoraLite® Plus 488 Anti-Mouse LAG-3/CD223 (C9B7W)

LAG-3/CD223 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

C9B7W

Cat no : CL488-65098

Print datasheet

Synonyms

CD223, LAG-3, Ly66, Lag3, LAG 3

验证数据展示

  • FC experiment of BALB/C mouse splenocytes using CL488-65098

    FC experiment of BALB/C mouse splenocytes using CL488-65098

    1X10^6 unstimulated or anti-CD3/CD28 stimulated (2 days) BALB/C mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2) and 0.5 ug CoraLite® Plus 488 Anti-Mouse CD223 (CL488-65098, Clone: C9B7W). Cells were not fixed.

经过测试的应用

Positive FC detected inAnti-CD3/CD28 treated mouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65098-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65098 targets LAG-3/CD223 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Murine CD223-Ig fusion protein 种属同源性预测
Host / Isotype Rat / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name lymphocyte-activation gene 3
Synonyms CD223, LAG-3, Ly66, Lag3, LAG 3
GenBank Accession NumberBC120591
Gene Symbol CD223
Gene ID (NCBI) 16768
RRIDAB_2919262
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 LAG-3/CD223 antibody CL488-65098Download protocol
Standard Protocols
Click here to view our Standard Protocols