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PE Anti-Mouse LAG-3/CD223 (C9B7W)

LAG-3/CD223 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

C9B7W

Cat no : PE-65098

Print datasheet

Synonyms

CD223, LAG-3, Ly66, Lag3, LAG 3

验证数据展示

  • FC experiment of mouse splenocytes using PE-65098

    FC experiment of mouse splenocytes using PE-65098

    1X10^6 anti-CD3/CD28 stimulated (3 days) mouse splenocytes were surface co-stained with CoraLite® Plus 488 Anti-Mouse CD3 and 0.2 ug PE Anti-Mouse CD223 (PE-65098, Clone: C9B7W) or 0.2 ug PE Mouse IgG1 Isotype Control. Cells were not fixed.

经过测试的应用

Positive FC detected inanti-CD3/CD28 stimulated mouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65098 targets LAG-3/CD223 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Murine CD223-Ig fusion protein 种属同源性预测
Host / Isotype Rat / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name lymphocyte-activation gene 3
Synonyms CD223, LAG-3, Ly66, Lag3, LAG 3
GenBank Accession NumberBC120591
Gene Symbol CD223
Gene ID (NCBI) 16768
RRIDAB_2883890
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ61790
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628).

实验方案

Product Specific Protocols
FC protocol for PE LAG-3/CD223 antibody PE-65098Download protocol
Standard Protocols
Click here to view our Standard Protocols