CoraLite® Plus 750-conjugated CD226 Recombinant antibody
CD226 Uni-rAbTM Recombinant Antibody for WB
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
230041E12
验证数据展示
经过测试的应用
| Positive WB detected in | human peripheral blood platelets |
For other applications, we recommend the unconjugated version of this antibody, 82991-3-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of CL750-82991-3 is CD226 Fusion Protein expressed in E. coli.
| Tested Applications | WB Application Description |
| Tested Reactivity | Human |
| Immunogen | Fusion Protein 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | CD226 molecule |
| Synonyms | CD226, CD226 antigen, CD226 molecule, DNAM 1, DNAM1, DNAX accessory molecule 1, PTA1, TLiSA1 |
| Calculated Molecular Weight | 336 aa, 39 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC074787 |
| Gene Symbol | CD226 |
| Gene ID (NCBI) | 10666 |
| ENSEMBL Gene ID | ENSG00000150637 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15762 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 CD226 antibody CL750-82991-3 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |