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验证数据展示

  • IF Staining of human tonsillitis using CL594-60208-2

    IF Staining of human tonsillitis using CL594-60208-2

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CoraLite®594 CD23 antibody (CL594-60208-2, Clone: 4C7C9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite®594-conjugated CD23 Monoclonal antibody

CD23 Monoclonal Antibody for IF-P
Cat No. CL594-60208-2

Print datasheet

CloneNo. 4C7C9

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF-P

Synonyms

C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD23 Ready-To-Use IHC Kit. CD23 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60208-2-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60208-2 targets CD23 in IF-P applications and shows reactivity with human, mouse samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse
Immunogen CD23 fusion protein Ag0425 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Synonyms C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2
Calculated Molecular Weight 321 aa, 36 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC064417
Gene Symbol CD23
Gene ID (NCBI) 2208
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06734
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.

实验方案

Product Specific Protocols
IF protocol for CL594 CD23 antibody CL594-60208-2Download protocol
Standard Protocols
Click here to view our Standard Protocols