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验证数据展示

  • WB analysis of Raji using 60208-2-Ig

    WB analysis of Raji using 60208-2-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 60208-2-Ig (CD23 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 60208-2-Ig

    IHC staining of human tonsillitis using 60208-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD23 antibody included
  • IHC staining of human tonsillitis using 60208-2-Ig

    IHC staining of human tonsillitis using 60208-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD23 antibody included
  • IF Staining of human tonsillitis using 60208-2-Ig

    IF Staining of human tonsillitis using 60208-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD23 antibody (60208-2-Ig, Clone: 4C7C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 60208-2-Ig

    IHC staining of human lymphoma using 60208-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60208-2-Ig (CD23 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD23 antibody included

CD23 Monoclonal antibody

CD23 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 60208-2-Ig

产品说明书

CloneNo. 4C7C9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD23 Ready-To-Use IHC Kit. CD23 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60208-2-Ig targets CD23 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 CD23 fusion protein Ag0425 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 Fc fragment of IgE, low affinity II, receptor for (CD23)
别名 C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2
计算分子量 321 aa, 36 kDa
观测分子量 40 kDa
GenBank蛋白编号BC064417
基因名称 CD23
Gene ID (NCBI) 2208
RRIDAB_11232221
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP06734
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.

实验方案

Product Specific Protocols
WB protocol for CD23 antibody 60208-2-IgDownload protocol
IHC protocol for CD23 antibody 60208-2-IgDownload protocol
IF protocol for CD23 antibody 60208-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols