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验证数据展示

  • WB analysis of Raji using 60208-2-Ig (same clone as 60208-2-PBS)

    WB analysis of Raji using 60208-2-Ig (same clone as 60208-2-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 60208-2-Ig (CD23 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD23 antibody included
  • IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD23 antibody included
  • IF Staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    IF Staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD23 antibody (60208-2-Ig, Clone: 4C7C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60208-2-Ig (CD23 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60208-2-Ig (CD23 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60208-2-Ig (CD23 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60208-2-Ig (CD23 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60208-2-Ig (CD23 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD23 antibody included
  • IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD23 antibody included
  • IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD23 antibody included
  • IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60208-2-Ig (CD23 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD23 antibody included
  • IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60208-2-Ig (CD23 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD23 antibody included
  • IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60208-2-Ig (CD23 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD23 antibody included
  • IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD23 antibody included
  • IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD23 antibody included
  • IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60208-2-Ig (CD23 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD23 antibody included
  • Cytometric bead array standard curve of MP50220-1

    Cytometric bead array standard curve of MP50220-1

    Cytometric bead array standard curve of MP50220-1, CD23,FCER2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60208-2-PBS. Detection antibody: 60208-3-PBS. Standard:Ag0425. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP50220-2

    Cytometric bead array standard curve of MP50220-2

    Cytometric bead array standard curve of MP50220-2, CD23,FCER2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60208-2-PBS. Detection antibody: 60208-4-PBS. Standard:Ag0425. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP50220-3

    Cytometric bead array standard curve of MP50220-3

    Cytometric bead array standard curve of MP50220-3, CD23,FCER2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60208-2-PBS. Detection antibody: 60208-1-PBS. Standard:Ag0425. Range: 0.391-100 ng/mL.

CD23 Monoclonal antibody, PBS Only (Capture)

CD23 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 60208-2-PBS

产品说明书

CloneNo. 4C7C9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

4C7C9, BLAST 2, BLAST-2, CD23A, CLEC4J

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CD23 Ready-To-Use IHC Kit. CD23 primary antibody included.

产品信息

60208-2-PBS targets CD23 as part of a matched antibody pair:

MP50220-1: 60208-2-PBS capture and 60208-3-PBS detection (validated in Cytometric bead array)

MP50220-2: 60208-2-PBS capture and 60208-4-PBS detection (validated in Cytometric bead array)

MP50220-3: 60208-2-PBS capture and 60208-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0425

Product name: Recombinant human CD23,FCER2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 48-248 aa of BC064417

Sequence: DTTQSLKQLEERAARNVSQVSKNLESHHGDQMAQKSQSTQISQELEELRAEQQRLKSQDLELSWNLNGLQADLSSFKSQELNERNEASDLLERLREEVTKLRMELQVSSGFVCNTCPEKWINFQRKCYYFGKGTKQWVHARYACDDMEGQLVSIHSPEEQDFLTKHASHTGSWIGLRNLDLKGEFIWVDGSHVDYSNWAPG

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 Fc fragment of IgE, low affinity II, receptor for (CD23)
别名 4C7C9, BLAST 2, BLAST-2, CD23A, CLEC4J
计算分子量 321 aa, 36 kDa
观测分子量 40 kDa
GenBank蛋白编号BC064417
基因名称 CD23
Gene ID (NCBI) 2208
RRIDAB_11232221
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP06734
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.

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