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Anti-Mouse B7-H3/CD276 (MJ18)

B7-H3/CD276 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

MJ18

Cat no : 65221-1-Ig

Print datasheet

Synonyms

CD276, CD276 antigen, B7RP 2, B7-H3, B7h3

验证数据展示

  • FC experiment of mouse peritoneal macrophages using 65221-1-Ig

    FC experiment of mouse peritoneal macrophages using 65221-1-Ig

    1X10^6 mouse peritoneal macrophages were surface stained with 1 ug Anti-Mouse CD276 (65221-1-Ig, Clone:MJ18) and FITC anti-rat IgG1 Antibody at dilution 1:100 (red), or unstained (blue). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse peritoneal macrophages
Planning an IF experiment? We recommend our CoraLite® Plus 405, CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65221-1-Ig targets B7-H3/CD276 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Mouse IgG2a Fc-CD276 (aa 1-242) fusion protein 种属同源性预测
Host / Isotype Rat / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD276 antigen
Synonyms CD276, CD276 antigen, B7RP 2, B7-H3, B7h3
GenBank Accession NumberBC019436
Gene Symbol Cd276
Gene ID (NCBI) 102657
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8VE98
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD276 (also known as B7-H3) is a type I transmembrane protein expressed on many tissues and cell types. CD276 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, CD276 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).

实验方案

Product Specific Protocols
FC protocol for B7-H3/CD276 antibody 65221-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols