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CoraLite®594 Anti-Mouse B7-H3/CD276 (MJ18)

B7-H3/CD276 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

mouse

Applications

FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

MJ18

Cat no : CL594-65221

Print datasheet

Synonyms

CD276, CD276 antigen, B7RP 2, B7-H3, B7h3

验证数据展示

  • FC experiment of BALB/C mouse peritoneal macrophages using CL594-65221

    FC experiment of BALB/C mouse peritoneal macrophages using CL594-65221

    1X10^6 BALB/C mouse peritoneal macrophages were surface stained with 0.5 ug CoraLite®594 Anti-Mouse CD276 (CL594-65221, Clone: MJ18), or 0.5 ug CoraLite®594 Rat IgG1 Isotype Control (HRPN) (CL594-65212, Clone: HRPN). Cells were not fixed.

经过测试的应用

Positive FC detected inBALB/C mouse peritoneal macrophages
For other applications, we recommend the unconjugated version of this antibody, 65221-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65221 targets B7-H3/CD276 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Mouse IgG2a Fc-CD276 (aa 1-242) fusion protein 种属同源性预测
Host / Isotype Rat / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD276 antigen
Synonyms CD276, CD276 antigen, B7RP 2, B7-H3, B7h3
GenBank Accession NumberBC019436
Gene Symbol Cd276
Gene ID (NCBI) 102657
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8VE98
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD276 (also known as B7-H3) is a type I transmembrane protein expressed on many tissues and cell types. CD276 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, CD276 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).

实验方案

Product Specific Protocols
FC protocol for CL594 B7-H3/CD276 antibody CL594-65221Download protocol
Standard Protocols
Click here to view our Standard Protocols