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Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody, PBS Only

CD30 Ligand/TNFSF8 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241831D9

Cat no : 98253-1-PBS

Print datasheet

Synonyms

TNFSF 8, CD30 L, TNFSF8, 241831D9, CD30LG

验证数据展示

  • FC experiment of human PBMCs using 98253-1-RR (same clone as 98253-1-PBS)

    FC experiment of human PBMCs using 98253-1-RR (same clone as 98253-1-PBS)

    1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. This data was developed using the same antibody clone with 98253-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98253-1-RR (same clone as 98253-1-PBS)

    FC experiment of human PBMCs using 98253-1-RR (same clone as 98253-1-PBS)

    1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98253-1-PBS in a different storage buffer formulation.

产品信息

98253-1-PBS targets CD30 Ligand/TNFSF8 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor (ligand) superfamily, member 8
Synonyms TNFSF 8, CD30 L, TNFSF8, 241831D9, CD30LG
Calculated Molecular Weight 234 aa, 26 kDa
GenBank Accession NumberBC093630
Gene Symbol TNFSF8
Gene ID (NCBI) 944
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP32971
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD30 Ligand (CD30L), also named as TNFSF8 or CD153, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) superfamily (PMID: 8391931). It is expressed on the surface of activated T cells, B cells, monocytes, macrophages, eosinophils, neutrophils, and mast cells (PMID: 26090498). CD30L interacts with its receptor, CD30, which is a cell surface protein expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin lymphoma and anaplastic large cell lymphomas (PMID: 28885612). The CD30/CD30L signaling is involved in pleiotropic downstream effects including differentiation, cell survival and death, NFkB activation, and production of cytokines (PMID: 38534788).