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验证数据展示

  • FC experiment of human PBMCs using 98253-1-RR

    FC experiment of human PBMCs using 98253-1-RR

    1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed.

  • FC experiment of human PBMCs using 98253-1-RR

    FC experiment of human PBMCs using 98253-1-RR

    1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98253-1-RR

    Affinity and Kinetic Characterization of 98253-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98253-1-RR against Human CD30 Ligand/TNFSF8 were performed. The affinity constant is below 1 pM.

Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody

CD30 Ligand/TNFSF8 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98253-1-RR

产品说明书

CloneNo. 241831D9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

TNFSF 8, CD30 L, TNFSF8, 241831D9, CD30LG

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inPMA and ionomycin treated human PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98253-1-RR targets CD30 Ligand/TNFSF8 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor (ligand) superfamily, member 8
别名 TNFSF 8, CD30 L, TNFSF8, 241831D9, CD30LG
计算分子量 234 aa, 26 kDa
GenBank蛋白编号BC093630
基因名称 TNFSF8
Gene ID (NCBI) 944
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP32971
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD30 Ligand (CD30L), also named as TNFSF8 or CD153, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) superfamily (PMID: 8391931). It is expressed on the surface of activated T cells, B cells, monocytes, macrophages, eosinophils, neutrophils, and mast cells (PMID: 26090498). CD30L interacts with its receptor, CD30, which is a cell surface protein expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin lymphoma and anaplastic large cell lymphomas (PMID: 28885612). The CD30/CD30L signaling is involved in pleiotropic downstream effects including differentiation, cell survival and death, NFkB activation, and production of cytokines (PMID: 38534788).

实验方案

Product Specific Protocols
FC protocol for CD30 Ligand/TNFSF8 antibody 98253-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols