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Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody

CD30 Ligand/TNFSF8 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241831D9

Cat no : 98253-1-RR

Print datasheet

Synonyms

TNFSF 8, CD30 L, TNFSF8, 241831D9, CD30LG

验证数据展示

  • FC experiment of human PBMCs using 98253-1-RR

    FC experiment of human PBMCs using 98253-1-RR

    1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed.

  • FC experiment of human PBMCs using 98253-1-RR

    FC experiment of human PBMCs using 98253-1-RR

    1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

经过测试的应用

Positive FC detected inPMA and ionomycin treated human PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98253-1-RR targets CD30 Ligand/TNFSF8 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor (ligand) superfamily, member 8
Synonyms TNFSF 8, CD30 L, TNFSF8, 241831D9, CD30LG
Calculated Molecular Weight 234 aa, 26 kDa
GenBank Accession NumberBC093630
Gene Symbol TNFSF8
Gene ID (NCBI) 944
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP32971
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD30 Ligand (CD30L), also named as TNFSF8 or CD153, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) superfamily (PMID: 8391931). It is expressed on the surface of activated T cells, B cells, monocytes, macrophages, eosinophils, neutrophils, and mast cells (PMID: 26090498). CD30L interacts with its receptor, CD30, which is a cell surface protein expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin lymphoma and anaplastic large cell lymphomas (PMID: 28885612). The CD30/CD30L signaling is involved in pleiotropic downstream effects including differentiation, cell survival and death, NFkB activation, and production of cytokines (PMID: 38534788).

实验方案

Product Specific Protocols
FC protocol for CD30 Ligand/TNFSF8 antibody 98253-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols