验证数据展示
经过测试的应用
Positive FC detected in | human PBMCs |
推荐稀释比
应用 | 推荐稀释比 |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-65268 targets CD31 in FC applications and shows reactivity with Human samples.
经测试应用 | FC Application Description |
经测试反应性 | Human |
免疫原 | N/A 种属同源性预测 |
宿主/亚型 | Mouse / IgG1, kappa |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | platelet/endothelial cell adhesion molecule |
别名 | CD31, EndoCAM, FLJ58394, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker |
计算分子量 | 83 kDa |
GenBank蛋白编号 | BC022512 |
基因名称 | CD31 |
Gene ID (NCBI) | 5175 |
ENSEMBL Gene ID | ENSG00000261371 |
RRID | AB_2935036 |
偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
最大激发/发射波长 | 654 nm / 674 nm |
形式 | Liquid |
纯化方式 | Affinity purification |
储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD31, also known as PECAM1 (Platelet endothelial cell adhesion molecule), is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. It is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, CD31 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 647 CD31 antibody CL647-65268 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |