验证数据展示
经过测试的应用
Positive WB detected in | TF-1 cells, Raji cells, U-937 cells, Mo7e cells, K-562 cells, THP-1 cells |
Positive IHC detected in | human liver cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | THP-1 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66529-1-Ig targets FCGR2A / CD32a in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | Human |
免疫原 | FCGR2A / CD32a fusion protein Ag25878 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | Fc fragment of IgG, low affinity IIa, receptor (CD32) |
别名 | CD32, CD32A, CDw32, Fc gamma RII a, Fc gamma RIIa, FCG2, FcGR, FCGR2, FCGR2A, FCGR2A1, FcRII a, IGFR2, IgG Fc receptor II a |
计算分子量 | 35 kDa |
观测分子量 | 42 kDa |
GenBank蛋白编号 | BC020823 |
基因名称 | CD32a |
Gene ID (NCBI) | 2212 |
RRID | AB_2881892 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P12318 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.
实验方案
Product Specific Protocols | |
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WB protocol for FCGR2A / CD32a antibody 66529-1-Ig | Download protocol |
IHC protocol for FCGR2A / CD32a antibody 66529-1-Ig | Download protocol |
IF protocol for FCGR2A / CD32a antibody 66529-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |