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FCGR2A / CD32a Monoclonal antibody

FCGR2A / CD32a Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D3F1

Cat no : 66529-1-Ig

Print datasheet

Synonyms

CD32, CD32A, CDw32, Fc gamma RII a, Fc gamma RIIa, FCG2, FcGR, FCGR2, FCGR2A, FCGR2A1, FcRII a, IGFR2, IgG Fc receptor II a

验证数据展示

  • WB analysis using 66529-1-Ig

    WB analysis using 66529-1-Ig

    Untreated and PNGase F-treated lysates of THP-1 cells and Mo7e cells were subjected to SDS PAGE followed by western blot with 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66529-1-Ig

    IHC staining of human liver cancer using 66529-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FCGR2A/CD32A antibody included
  • IHC staining of human liver cancer using 66529-1-Ig

    IHC staining of human liver cancer using 66529-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FCGR2A/CD32A antibody included
  • IF Staining of THP-1 using 66529-1-Ig

    IF Staining of THP-1 using 66529-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using FCGR2A / CD32a antibody (66529-1-Ig, Clone: 1D3F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inTF-1 cells, Raji cells, U-937 cells, Mo7e cells, K-562 cells, THP-1 cells
Positive IHC detected inhuman liver cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells
Planning an IHC experiment? We recommend our IHCeasy FCGR2A/CD32A Ready-To-Use IHC Kit. FCGR2A/CD32A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66529-1-Ig targets FCGR2A / CD32a in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen FCGR2A / CD32a fusion protein Ag25878 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name Fc fragment of IgG, low affinity IIa, receptor (CD32)
Synonyms CD32, CD32A, CDw32, Fc gamma RII a, Fc gamma RIIa, FCG2, FcGR, FCGR2, FCGR2A, FCGR2A1, FcRII a, IGFR2, IgG Fc receptor II a
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC020823
Gene Symbol CD32a
Gene ID (NCBI) 2212
RRIDAB_2881892
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

实验方案

Product Specific Protocols
WB protocol for FCGR2A / CD32a antibody 66529-1-IgDownload protocol
IHC protocol for FCGR2A / CD32a antibody 66529-1-IgDownload protocol
IF protocol for FCGR2A / CD32a antibody 66529-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols