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验证数据展示

  • IF Staining of THP-1 using CL488-66529

    IF Staining of THP-1 using CL488-66529

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CoraLite® Plus 488 FCGR2A / CD32a antibody (CL488-66529, Clone: 1D3F1 ) at dilution of 1:50.

CoraLite® Plus 488-conjugated FCGR2A / CD32a Monoclonal antibody

FCGR2A / CD32a Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-66529

产品说明书

CloneNo. 1D3F1

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

IF/ICC, IF-P

别名

CD32, CD32A, CDw32, Fc gamma RII a, Fc gamma RIIa, FCG2, FcGR, FCGR2, FCGR2A, FCGR2A / CD32a, FCGR2A1, FcRII a, IGFR2, IgG Fc receptor II a

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inTHP-1 cells, human tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy FCGR2A/CD32A Ready-To-Use IHC Kit. FCGR2A/CD32A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66529-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66529 targets FCGR2A / CD32a in IF/ICC, IF-P applications and shows reactivity with Human samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 Human
免疫原 FCGR2A / CD32a fusion protein Ag25878 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIa, receptor (CD32)
别名 CD32, CD32A, CDw32, Fc gamma RII a, Fc gamma RIIa, FCG2, FcGR, FCGR2, FCGR2A, FCGR2A / CD32a, FCGR2A1, FcRII a, IGFR2, IgG Fc receptor II a
计算分子量 35 kDa
GenBank蛋白编号BC020823
基因名称 CD32a
Gene ID (NCBI) 2212
RRIDAB_2883350
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12318
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FCGR2A / CD32a antibody CL488-66529Download protocol
Standard Protocols
Click here to view our Standard Protocols