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Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody, PBS Only

CD324 (E-cadherin) Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240159B4

Cat no : 98030-2-PBS

Print datasheet

Synonyms

E-cadherin, CD324, CAM 120/80, Cadherin-1, Cadherin 1

验证数据展示

  • FC experiment of MCF-7 using 98030-2-RR (same clone as 98030-2-PBS)

    FC experiment of MCF-7 using 98030-2-RR (same clone as 98030-2-PBS)

    1x10^6 MCF-7 cells were surface stained with 0.25 ug Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody (98030-2-RR, Clone:240159B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells should be stained prior to fixation. This data was developed using the same antibody clone with 98030-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98030-2-RR

    Affinity and Kinetic Characterization of 98030-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98030-2-RR against Human E-cadherin were performed. The affinity constant is 0.204 nM.

Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.

产品信息

The immunogen of 98030-2-PBS is CD324 (E-cadherin) Fusion Protein expressed in E. coli.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen CD324 (E-cadherin) fusion protein Ag14973 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Synonyms E-cadherin, CD324, CAM 120/80, Cadherin-1, Cadherin 1
Calculated Molecular Weight 882 aa, 97 kDa
GenBank Accession NumberBC141838
Gene Symbol E-cadherin
Gene ID (NCBI) 999
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP12830
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.