CoraLite® Plus 750 Anti-Human CD33 (WM53)
CD33 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human
Applications
FC
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
WM53
验证数据展示
经过测试的应用
Positive FC detected in | human PBMCs |
For other applications, we recommend the unconjugated version of this antibody, 65272-1-Ig
推荐稀释比
Application | Dilution |
---|---|
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL750-65272 targets CD33 in FC applications and shows reactivity with Human samples.
Tested Applications | FC Application Description |
Tested Reactivity | Human |
Immunogen | N/A 种属同源性预测 |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Full Name | CD33 molecule |
Synonyms | CD33, CD33 molecule, FLJ00391, gp67, p67, SIGLEC 3, SIGLEC3 |
Calculated Molecular Weight | 364 aa, 40 kDa |
GenBank Accession Number | BC028152 |
Gene Symbol | CD33 |
Gene ID (NCBI) | 945 |
Conjugate | CoraLite® Plus 750 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.
实验方案
Product Specific Protocols | |
---|---|
FC protocol for CL Plus 750 CD33 antibody CL750-65272 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |