• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 594 Anti-Human CD33 (WM53)

CD33 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

WM53

Cat no : CL594-65272

Print datasheet

Synonyms

SIGLEC3, SIGLEC 3, Sialic acid-binding Ig-like lectin 3, p67, gp67

验证数据展示

  • FC experiment of human PBMCs using CL594-65272

    FC experiment of human PBMCs using CL594-65272

    1x10^6 FC receptor blocked human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD33 (CL594-65272, Clone: WM53) (red) or CoraLite®594 Mouse IgG1 Isotype Control (blue). Cells were not fixed. Monocytes were gated.

  • FC experiment of human PBMCs using CL594-65272

    FC experiment of human PBMCs using CL594-65272

    1x10^6 FC receptor blocked human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD33 (CL594-65272, Clone: WM53) or CoraLite®594 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes and monocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65272-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65272 targets CD33 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD33 molecule
Synonyms SIGLEC3, SIGLEC 3, Sialic acid-binding Ig-like lectin 3, p67, gp67
Calculated Molecular Weight 364 aa, 40 kDa
GenBank Accession NumberBC028152
Gene Symbol CD33
Gene ID (NCBI) 945
Conjugate CoraLite® Plus 594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths594 nm / 615 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.

实验方案

Product Specific Protocols
FC protocol for CL Plus 594 CD33 antibody CL594-65272Download protocol
Standard Protocols
Click here to view our Standard Protocols