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CoraLite® Plus 647 Anti-Mouse CD357 (GITR) (DTA-1)

CD357 (GITR) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

DTA-1

Cat no : CL647-65102

Print datasheet

Synonyms

AITR, CD357, CD357 (GITR), Gitr, Tnfrsf18

验证数据展示

  • FC experiment of mouse splenocytes using CL647-65102

    FC experiment of mouse splenocytes using CL647-65102

    1X10^6 mouse splenocytes were surface stained with PE-Anti-Mouse CD4 (PE-65104, Clone: GK1.5), CoraLite®488-Conjugated Anti-Mouse CD25 (CL488-65137, Clone: PC61.5) and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD357 (GITR) (CL647-65102, Clone: DTA-1). Cells were not fixed. CD4+ lymphocytes were gated for analysis of CD25 and CD357 (GITR) staining.

经过测试的应用

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65102-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65102 targets CD357 (GITR) in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen Mouse CD25+ CD4+ T cells 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 18
Synonyms AITR, CD357, CD357 (GITR), Gitr, Tnfrsf18
GenBank Accession NumberBC146517
Gene Symbol CD357
Gene ID (NCBI) 21936
RRIDAB_2883680
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Glucocorticoid-induced TNFR-related protein (GITR), also known as CD357 or TNFRSF18, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is expressed constitutively at high levels in T regulatory cells (Treg cells) and plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells (PMID: 11812990). It is expressed at low levels on resting responder T cells. The expression of GITR on T cells can be upregulated upon activation (PMID: 15770698). GITR is activated by GITR ligand (GITRL) which is mainly expressed on APC. GITR-GITRL interactions could co-stimulate both responder T-cell functions and the suppressive functions of Treg cells (PMID: 16868552).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD357 (GITR) antibody CL647-65102Download protocol
Standard Protocols
Click here to view our Standard Protocols