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CoraLite® Plus 750 Anti-Mouse CD357 (GITR) (DTA-1)

CD357 (GITR) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

DTA-1

Cat no : CL750-65102

Print datasheet

Synonyms

AITR, Gitr, Tnfrsf18

验证数据展示

  • FC experiment of mouse splenocytes using CL750-65102

    FC experiment of mouse splenocytes using CL750-65102

    1X10^6 mouse splenocytes were surface co-stained CoraLite® Plus 488 Anti-Mouse CD4 and with 0.5 ug CoraLite® Plus 750 Anti-Mouse CD357 (GITR) (CL750-65102, Clone: DTA-1) or 0.5 ug Isotype Control. Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65102-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-65102 targets CD357 (GITR) in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen Mouse CD25+ CD4+ T cells 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 18
Synonyms AITR, Gitr, Tnfrsf18
GenBank Accession NumberBC146517
Gene Symbol CD357
Gene ID (NCBI) 21936
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Glucocorticoid-induced TNFR-related protein (GITR), also known as CD357 or TNFRSF18, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is expressed constitutively at high levels in T regulatory cells (Treg cells) and plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells (PMID: 11812990). It is expressed at low levels on resting responder T cells. The expression of GITR on T cells can be upregulated upon activation (PMID: 15770698). GITR is activated by GITR ligand (GITRL) which is mainly expressed on APC. GITR-GITRL interactions could co-stimulate both responder T-cell functions and the suppressive functions of Treg cells (PMID: 16868552).

实验方案

Product Specific Protocols
FC protocol for CL Plus 750 CD357 (GITR) antibody CL750-65102Download protocol
Standard Protocols
Click here to view our Standard Protocols