CoraLite® Plus 488 Anti-Mouse CD4 (RM4-5)

CD4 Monoclonal Antibody for IF/ICC, FC
Cat No. CL488-65141

产品说明书

CloneNo. RM4-5

宿主/亚型

Rat / IgG2a, kappa

种属反应性

Mouse and More (1)

应用

IF/ICC, FC and More (1)

Cd4, CD4 antigen, L3T4, Ly 4

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65141-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

CL488-65141 targets CD4 in IF/ICC, FC applications and shows reactivity with Mouse samples.

经测试应用 IF/ICC, FC Application Description
文献引用应用IF
经测试反应性 Mouse
文献引用反应性mouse
免疫原 BALB/c mouse thymocytes 种属同源性预测
宿主/亚型 Rat / IgG2a, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD4 antigen
别名 Cd4, CD4 antigen, L3T4, Ly 4
GenBank蛋白编号BC039137
基因名称 Cd4
Gene ID (NCBI) 12504
RRIDAB_2883201
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD4 antibody CL488-65141Download protocol
FC protocol for CL Plus 488 CD4 antibody CL488-65141Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Nat Commun

In vivo self-assembled siRNA as a modality for combination therapy of ulcerative colitis

Authors - Xinyan Zhou
mouseIF

Cell Commun Signal

Tumorous IRE1α facilitates CD8+T cells-dependent anti-tumor immunity and improves immunotherapy efficacy in melanoma

Authors - Yuqi Yang
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