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CoraLite® Plus 555 Anti-Mouse CD4 (RM4-5)

CD4 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

RM4-5

Cat no : CL555-65141

Print datasheet

Synonyms

Cd4, CD4 antigen, L3T4, Ly 4

验证数据展示

  • FC experiment of mouse splenocytes using CL555-65141

    FC experiment of mouse splenocytes using CL555-65141

    1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 and 0.5 ug CoraLite® Plus 555 Anti-Mouse CD4 (CL555-65141, Clone: RM4-5) or 0.5 ug Isotype Control. Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65141-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-65141 targets CD4 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen BALB/c mouse thymocytes 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name CD4 antigen
Synonyms Cd4, CD4 antigen, L3T4, Ly 4
GenBank Accession NumberBC039137
Gene Symbol Cd4
Gene ID (NCBI) 12504
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 CD4 antibody CL555-65141Download protocol
Standard Protocols
Click here to view our Standard Protocols