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CoraLite® Plus 488-conjugated CD44 Monoclonal antibody
CD44 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
human, pig
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
6F4H2
验证数据展示
经过测试的应用
Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy CD44 Ready-To-Use IHC Kit. CD44 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60224-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-60224 targets CD44 in IF/ICC applications and shows reactivity with human, pig samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human, pig |
Immunogen | CD44 fusion protein Ag7633 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | CD44 molecule (Indian blood group) |
Synonyms | ECMR-III, ECMR III, CSPG8, CDW44, CD44 antigen |
Calculated Molecular Weight | 742 aa, 82 kDa |
Observed Molecular Weight | 80-95 kDa |
GenBank Accession Number | BC004372 |
Gene Symbol | CD44 |
Gene ID (NCBI) | 960 |
ENSEMBL Gene ID | ENSG00000026508 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P16070 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 CD44 antibody CL488-60224 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |