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验证数据展示

  • IF Staining of human lung cancer using CL594-60224

    IF Staining of human lung cancer using CL594-60224

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®594 CD44 antibody (CL594-60224, Clone: 6F4H2 ) at dilution of 1:200.

  • IF Staining of human lung cancer using CL594-60224

    IF Staining of human lung cancer using CL594-60224

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®594 CD44 antibody (CL594-60224, Clone: 6F4H2 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated CD44 Monoclonal antibody

CD44 Monoclonal Antibody for IF-P
Cat No. CL594-60224

产品说明书

CloneNo. 6F4H2

宿主/亚型

Mouse / IgG2a

种属反应性

human, pig

应用

IF-P

别名

ECMR-III, ECMR III, CSPG8, CDW44, CD44 antigen

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman lung cancer tissue
Planning an IHC experiment? We recommend our IHCeasy CD44 Ready-To-Use IHC Kit. CD44 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60224-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60224 targets CD44 in IF-P applications and shows reactivity with human, pig samples.

经测试应用 IF-P Application Description
经测试反应性 human, pig
免疫原 CD44 fusion protein Ag7633 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 CD44 molecule (Indian blood group)
别名 ECMR-III, ECMR III, CSPG8, CDW44, CD44 antigen
计算分子量 742 aa, 82 kDa
GenBank蛋白编号BC004372
基因名称 CD44
Gene ID (NCBI) 960
ENSEMBL Gene IDENSG00000026508
RRIDAB_2883445
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP16070
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

实验方案

Product Specific Protocols
IF protocol for CL594 CD44 antibody CL594-60224Download protocol
Standard Protocols
Click here to view our Standard Protocols