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验证数据展示

  • FC experiment of mouse splenocytes using PE-65117

    FC experiment of mouse splenocytes using PE-65117

    1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.25 ug PE Anti-Mouse CD44 (PE-65117, Clone:IM7) or 0.25 ug PE Rat IgG2b Isotype Control (LTF-2). Cells were not fixed.

PE Anti-Mouse CD44 (IM7)

CD44 Monoclonal Antibody for FC
Cat No. PE-65117

Print datasheet

CloneNo. IM7

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Synonyms

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  PE
Size: 

-/ -

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经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65117 targets CD44 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen Dexamethasone-induced myeloid leukemia M1 cells 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name CD44 antigen
Synonyms CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I
GenBank Accession NumberBC051388
Gene Symbol Cd44
Gene ID (NCBI) 12505
RRIDAB_2883906
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP15379
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

实验方案

Product Specific Protocols
FC protocol for PE CD44 antibody PE-65117Download protocol
Standard Protocols
Click here to view our Standard Protocols