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验证数据展示

  • FC experiment of C57BL/c mouse splenocytes using 65117-1-Ig

    FC experiment of C57BL/c mouse splenocytes using 65117-1-Ig

    1X10^6 C57BL/c mouse splenocytes were surface co-stained with APC Anti-Mouse CD3 (17A2), and 0.5 ug Anti-Mouse CD44 (65117-1-Ig, Clone: IM7) or 0.5 ug isotype control, and FITC anti-rat IgG2b Antibody at dilution 1:100. Cells were not fixed.

  • IF Staining of HeLa using 65117-1-Ig

    IF Staining of HeLa using 65117-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CD44 antibody (65117-1-Ig, Clone: IM7 ) at dilution of 1:400 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).

Anti-Mouse CD44 (IM7)

CD44 Monoclonal Antibody for IF/ICC, FC
Cat No. 65117-1-Ig

Print datasheet

CloneNo. IM7

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Human, Mouse

Applications

IF/ICC, FC

Synonyms

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 
500 μg

¥1190/ 500 μg

浓度: 0.5 mg/ml

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:400
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65117-1-Ig targets CD44 in IF/ICC, FC applications and shows reactivity with Human, Mouse samples.

Tested Applications IF/ICC, FC Application Description
Tested Reactivity Human, Mouse
Immunogen Dexamethasone-induced myeloid leukemia M1 cells 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name CD44 antigen
Synonyms CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I
GenBank Accession NumberBC051388
Gene Symbol Cd44
Gene ID (NCBI) 12505
RRIDAB_2918413
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15379
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

实验方案

Product Specific Protocols
IF protocol for CD44 antibody 65117-1-IgDownload protocol
FC protocol for CD44 antibody 65117-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols