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验证数据展示

  • FC experiment of human peripheral blood lymphocytes using 65082-1-Ig

    FC experiment of human peripheral blood lymphocytes using 65082-1-Ig

    1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug Anti-Human CD45 (65082-1-Ig, Clone: 2D1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.5 ug isotype control and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

  • IF Staining of mouse spleen using 65082-1-Ig

    IF Staining of mouse spleen using 65082-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using CD45 antibody (65082-1-Ig, Clone: 2D1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

Anti-Human CD45 (2D1)

CD45 Monoclonal Antibody for IF-Fro, FC
Cat No. 65082-1-Ig

产品说明书

CloneNo. 2D1

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

IF-Fro, FC

别名

L CA, GP180, EC:3.1.3.48, CD45R, B220

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF-Fro detected inmouse spleen tissue
Positive FC detected inhuman peripheral blood lymphocytes
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite® Plus 594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-FROIF-FRO : 1:125-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65082-1-Ig targets CD45 in IF-Fro, FC applications and shows reactivity with human samples.

经测试应用 IF-Fro, FC Application Description
经测试反应性 human
免疫原 Human PBMC 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C
别名 L CA, GP180, EC:3.1.3.48, CD45R, B220
GenBank蛋白编号BC014239
基因名称 CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_2918385
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

实验方案

Product Specific Protocols
IF protocol for CD45 antibody 65082-1-IgDownload protocol
FC protocol for CD45 antibody 65082-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols