CoraLite® Plus 488 Anti-Human CD45 (2D1)
CD45 Monoclonal Antibody for IF/ICC, FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
human
Applications
IF/ICC, FC and More (1)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2D1
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | human peripheral blood mononuclear cells |
| Positive FC detected in | human PBMCs |
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65082-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-65082 targets CD45 in IF/ICC, FC applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, FC Application Description |
| Cited Applications | IF |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Human PBMC 种属同源性预测 |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | protein tyrosine phosphatase, receptor type, C |
| Synonyms | L CA, GP180, EC:3.1.3.48, CD45R, B220 |
| GenBank Accession Number | BC014239 |
| Gene Symbol | CD45 |
| Gene ID (NCBI) | 5788 |
| ENSEMBL Gene ID | ENSG00000081237 |
| RRID | AB_2883166 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD45 antibody CL488-65082 | Download protocol |
| FC protocol for CL Plus 488 CD45 antibody CL488-65082 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Anal Chim Acta Layer-by-layer fabrication of alginate/polyethyleneimine multilayer on magnetic interface with enhanced efficiency in immuno-capturing circulating tumor cells |
