验证数据展示
经过测试的应用
Positive FC detected in | Human peripheral blood lymphocytes, Raji cells |
推荐稀释比
应用 | 推荐稀释比 |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
PE-65082 targets CD45 in IF, FC applications and shows reactivity with human samples.
经测试应用 | FC Application Description |
文献引用应用 | IF |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | Human PBMC 种属同源性预测 |
宿主/亚型 | Mouse / IgG1, kappa |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | protein tyrosine phosphatase, receptor type, C |
别名 | L CA, GP180, EC:3.1.3.48, CD45R, B220 |
GenBank蛋白编号 | BC014239 |
基因名称 | CD45 |
Gene ID (NCBI) | 5788 |
ENSEMBL Gene ID | ENSG00000081237 |
RRID | AB_2883877 |
偶联类型 | PE Fluorescent Dye |
最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
形式 | Liquid |
纯化方式 | Affinity purification |
UNIPROT ID | P08575 |
储存缓冲液 | PBS with 0.09% sodium azide and 0.1% gelatin , pH 7.2 |
储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).
实验方案
Product Specific Protocols | |
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FC protocol for PE CD45 antibody PE-65082 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |