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Anti-Mouse CD45 (30-F11)

CD45 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

30-F11

Cat no : 65087-1-Ig

Print datasheet

Synonyms

Ptprc, Leukocyte common antigen, LCA, B220, 30-F11

验证数据展示

  • FC experiment of mouse splenocytes using 65087-1-Ig

    FC experiment of mouse splenocytes using 65087-1-Ig

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD45 (30-F11) (65087-1-Ig, Clone: 30-F11) or 0.25 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2), and FITC anti-rat IgG2b Antibody. Cells were not fixed.

  • FC experiment of mouse splenocytes using 65087-1-Ig

    FC experiment of mouse splenocytes using 65087-1-Ig

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD45 (30-F11) (65087-1-Ig, Clone:30-F11) or 0.25 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2), and FITC anti-rat IgG2b Antibody. Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65087-1-Ig targets CD45 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Mouse thymus or spleen 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms Ptprc, Leukocyte common antigen, LCA, B220, 30-F11
GenBank Accession NumberBC028512
Gene Symbol CD45
Gene ID (NCBI) 19264
RRIDAB_2918390
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP06800
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

实验方案

Product Specific Protocols
FC protocol for CD45 antibody 65087-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols