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验证数据展示

  • FC experiment of mouse splenocytes using 65087-1-Ig

    FC experiment of mouse splenocytes using 65087-1-Ig

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD45 (30-F11) (65087-1-Ig, Clone: 30-F11) or 0.25 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2), and FITC anti-rat IgG2b Antibody. Cells were not fixed.

  • FC experiment of mouse splenocytes using 65087-1-Ig

    FC experiment of mouse splenocytes using 65087-1-Ig

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD45 (30-F11) (65087-1-Ig, Clone:30-F11) or 0.25 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2), and FITC anti-rat IgG2b Antibody. Cells were not fixed.

Anti-Mouse CD45 (30-F11)

CD45 Monoclonal Antibody for FC
Cat No. 65087-1-Ig

产品说明书

CloneNo. 30-F11

宿主/亚型

Rat / IgG2b, kappa

种属反应性

mouse

应用

FC

别名

Ptprc, Leukocyte common antigen, LCA, B220, 30-F11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65087-1-Ig targets CD45 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原 Mouse thymus or spleen 种属同源性预测
宿主/亚型 Rat / IgG2b, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C
别名 Ptprc, Leukocyte common antigen, LCA, B220, 30-F11
GenBank蛋白编号BC028512
基因名称 CD45
Gene ID (NCBI) 19264
RRIDAB_2918390
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP06800
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

实验方案

Product Specific Protocols
FC protocol for CD45 antibody 65087-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols