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Rat IgG2b Isotype Control (LTF-2)

IgG2b Isotype Control Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

and More (1)

Applications

FC and More (1)

Conjugate

Unconjugated

CloneNo.

LTF-2

Cat no : 65211-1-Ig

Print datasheet

Synonyms

Rat IgG2b Isotype Control, IgG2b

验证数据展示

  • FC experiment of mouse splenocytes using 65211-1-Ig

    FC experiment of mouse splenocytes using 65211-1-Ig

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2) (red) or Anti-Mouse CD4 (GK1.5) (65104-1-Ig, Clone: GK1.5) (green), and FITC anti-rat IgG2b Antibody. Cells were not fixed.

经过测试的应用

Positive FC detected in
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite® Plus 555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

65211-1-Ig targets IgG2b Isotype Control in IF, FC applications and shows reactivity with samples.

Tested Applications FC Application Description
Cited ApplicationsIF
Tested Reactivity
Cited Reactivitymouse
Immunogen N/A 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name IgG2b Isotype Control
Synonyms Rat IgG2b Isotype Control, IgG2b
Gene Symbol
Gene ID (NCBI)
RRIDAB_2881487
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

The LTF-2 immunoglobulin is useful as an isotype-matched control. The LTF-2 immunolglobulin has an unknown binding specificity and is used as an isotype control for rat IgG2b antibodies. This antibody has been quality-tested for flow cytometry as negative control.

实验方案

Product Specific Protocols
FC protocol for IgG2b Isotype Control antibody 65211-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Nat Commun

The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression.

Authors - Xinzhi Li
View Article
mouseIF

Front Endocrinol (Lausanne)

Ovaries of estrogen receptor 1-deficient mice show iron overload and signs of aging

Authors - Sarah K Schröder
View Article