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验证数据展示

  • FC experiment of mouse splenocytes using CL647-65087

    FC experiment of mouse splenocytes using CL647-65087

    1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD45 (CL647-65087, Clone: 30-F11) (red) or isotype control antibody (blue). Cells were not fixed.

  • IF Staining of mouse splenocytes using CL647-65087

    IF Staining of mouse splenocytes using CL647-65087

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD45 (CL647-65087, Clone: 30-F11) at dilution of 1:400.

CoraLite® Plus 647 Anti-Mouse CD45 (30-F11)

CD45 Monoclonal Antibody for IF/ICC, FC
Cat No. CL647-65087

Print datasheet

CloneNo. 30-F11

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

IF/ICC, FC

Synonyms

Ptprc, Leukocyte common antigen, LCA, B220, 30-F11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65087-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65087 targets CD45 in IF/ICC, FC applications and shows reactivity with mouse samples.

Tested Applications IF/ICC, FC Application Description
Tested Reactivity mouse
Immunogen Mouse thymus or spleen 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms Ptprc, Leukocyte common antigen, LCA, B220, 30-F11
GenBank Accession NumberBC028512
Gene Symbol CD45
Gene ID (NCBI) 19264
RRIDAB_2883673
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP06800
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 CD45 antibody CL647-65087Download protocol
Standard Protocols
Click here to view our Standard Protocols