CoraLite® Plus 647 Anti-Rat CD45RA (OX-33)
CD45RA Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
rat
Applications
FC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
OX-33
验证数据展示
经过测试的应用
| Positive FC detected in | rat splenocytes cells |
For other applications, we recommend the unconjugated version of this antibody, 65234-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-65234 targets CD45RA in FC applications and shows reactivity with rat samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | rat |
| Immunogen | Leukocyte common antigen purified from rat splenocytes 种属同源性预测 |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | protein tyrosine phosphatase, receptor type, C |
| Synonyms | Lca, Ptprc, CD45, RT7, OX-33 |
| Calculated Molecular Weight | 143 kDa |
| GenBank Accession Number | XM_006249910 |
| Gene Symbol | CD45 |
| Gene ID (NCBI) | 24699 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P04157 |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 CD45RA antibody CL647-65234 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
