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PE Anti-Rat CD45RA (OX-33)

CD45RA Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

rat

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

OX-33

Cat no : PE-65234

Print datasheet

Synonyms

Lca, Ptprc, CD45, RT7, OX-33

验证数据展示

  • FC experiment of rat splenocytes using PE-65234

    FC experiment of rat splenocytes using PE-65234

    1x10^6 rat splenocytes were surface stained with 0.1 ug PE Anti-Rat CD45RA (OX-33) (PE-65234, Clone: OX-33) or 0.1 ug PE Mouse IgG1 Isotype Control (MOPC-21) (PE-65124, Clone: MOPC-21), and APC Anti-Rat CD4 (OX-35) (APC-65179, Clone: OX-35). Cells were not fixed.

经过测试的应用

Positive FC detected inrat splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65234 targets CD45RA in FC applications and shows reactivity with rat samples.

Tested Applications FC Application Description
Tested Reactivity rat
Immunogen Leukocyte common antigen purified from rat splenocytes 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms Lca, Ptprc, CD45, RT7, OX-33
Calculated Molecular Weight143 kDa
GenBank Accession NumberXM_006249910
Gene Symbol CD45
Gene ID (NCBI) 24699
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodN/A
UNIPROT IDP04157
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

实验方案

Product Specific Protocols
FC protocol for PE CD45RA antibody PE-65234Download protocol
Standard Protocols
Click here to view our Standard Protocols