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Anti-Human CD49b (AK7)

CD49b Monoclonal Antibody for FC, ELISA

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC, ELISA

Conjugate

Unconjugated

CloneNo.

AK7

Cat no : 65252-1-Ig

Print datasheet

Synonyms

BR, CD49B, Collagen receptor, GPIa, Integrin alpha 2, ITGA2, VLA 2, VLA 2 subunit alpha, VLAA2

验证数据展示

  • FC experiment of human PBMCs using 65252-1-Ig

    FC experiment of human PBMCs using 65252-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD49b (65252-1-Ig, Clone:AK7) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:500. Cells were not fixed. Monocytes were gated.

  • FC experiment of human PBMCs using 65252-1-Ig

    FC experiment of human PBMCs using 65252-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD49b (65252-1-Ig, Clone:AK7) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:500. Cells were not fixed. Monocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65252-1-Ig targets CD49b in FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, ELISA Application Description
Tested Reactivity Human
Immunogen Human platelets 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
Synonyms BR, CD49B, Collagen receptor, GPIa, Integrin alpha 2, ITGA2, VLA 2, VLA 2 subunit alpha, VLAA2
Calculated Molecular Weight 126 kDa
GenBank Accession NumberNM_002203
Gene Symbol Integrin alpha 2
Gene ID (NCBI) 3673
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD49b, also known as alpha2 integrin, is a type I transmembrane glycoprotein which forms an adhesion molecule VLA-2 with beta1 integrin. CD49b is a receptor for many (extracellular) matrix and non-matrix molecules (PMID: 23847623). CD49b is expressed on numerous cell types including platelets, activated T cells, B cells, and monocytes. CD49b activation skews NK cell effector function to a phenotype that exhibits elevated IFN-γ production with a concomitant inhibition of motility and cytolytic function (PMID: 32971468, 32762681). The Anti-Human CD49b antibody, clone AK7, is derived from Human platelets.

实验方案

Product Specific Protocols
FC protocol for CD49b antibody 65252-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols