验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL405-65252 targets CD49b in FC applications and shows reactivity with Human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | Human |
| 免疫原 | Human platelets 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor) |
| 别名 | BR, CD49B, Collagen receptor, GPIa, Integrin alpha 2, ITGA2, VLA 2, VLA 2 subunit alpha, VLAA2 |
| 计算分子量 | 126 kDa |
| GenBank蛋白编号 | NM_002203 |
| 基因名称 | Integrin alpha 2 |
| Gene ID (NCBI) | 3673 |
| RRID | AB_2934391 |
| 偶联类型 | CoraLite® Plus 405 Fluorescent Dye |
| 最大激发/发射波长 | 399 nm / 422 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD49b, also known as alpha2 integrin, is a type I transmembrane glycoprotein which forms an adhesion molecule VLA-2 with beta1 integrin. CD49b is a receptor for many (extracellular) matrix and non-matrix molecules (PMID: 23847623). CD49b is expressed on numerous cell types including platelets, activated T cells, B cells, and monocytes. CD49b activation skews NK cell effector function to a phenotype that exhibits elevated IFN-γ production with a concomitant inhibition of motility and cytolytic function (PMID: 32971468, 32762681). The Anti-Human CD49b antibody, clone AK7, is derived from Human platelets.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 405 CD49b antibody CL405-65252 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
