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Cardinal Red™ Anti-Human CD49b (AK7)

CD49b Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Cardinal Red™ Fluorescent Dye

CloneNo.

AK7

Cat no : CR-65252

Print datasheet

Synonyms

BR, CD49B, Collagen receptor, GPIa, Integrin alpha 2, ITGA2, VLA 2, VLA 2 subunit alpha, VLAA2

验证数据展示

  • FC experiment of human PBMCs using CR-65252

    FC experiment of human PBMCs using CR-65252

    1x10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD49b (AK7) (CR-65252) or Cardinal Red™ Mouse IgG1 Isotype Control. Cells were treated with FC receptor block prior to staining. Cells were not fixed. Monocytes were gated.

  • FC experiment of human PBMCs using CR-65252

    FC experiment of human PBMCs using CR-65252

    1x10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD49b (AK7) (CR-65252) or Cardinal Red™ Mouse IgG1 Isotype Control. Cells were treated with FC receptor block prior to staining. Cells were not fixed. Monocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CR-65252 targets CD49b in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human platelets 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
Synonyms BR, CD49B, Collagen receptor, GPIa, Integrin alpha 2, ITGA2, VLA 2, VLA 2 subunit alpha, VLAA2
Calculated Molecular Weight 126 kDa
GenBank Accession NumberNM_002203
Gene Symbol Integrin alpha 2
Gene ID (NCBI) 3673
Conjugate Cardinal Red™ Fluorescent Dye
Excitation/Emission Maxima Wavelengths592 nm / 611 nm
Form Liquid
Purification MethodN/A
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD49b, also known as alpha2 integrin, is a type I transmembrane glycoprotein which forms an adhesion molecule VLA-2 with beta1 integrin. CD49b is a receptor for many (extracellular) matrix and non-matrix molecules (PMID: 23847623). CD49b is expressed on numerous cell types including platelets, activated T cells, B cells, and monocytes. CD49b activation skews NK cell effector function to a phenotype that exhibits elevated IFN-γ production with a concomitant inhibition of motility and cytolytic function (PMID: 32971468, 32762681). The Anti-Human CD49b antibody, clone AK7, is derived from Human platelets.

实验方案

Product Specific Protocols
FC protocol for Cardinal Red™ CD49b antibody CR-65252Download protocol
Standard Protocols
Click here to view our Standard Protocols