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Anti-Mouse CD68 Rabbit Recombinant Antibody

CD68 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

230504G5

Cat no : 98029-1-RR

Print datasheet

Synonyms

Scard1, gp110, CD68 antigen, 230504G5

验证数据展示

  • FC experiment of mouse peritoneal macrophages using 98029-1-RR

    FC experiment of mouse peritoneal macrophages using 98029-1-RR

    1x10^6 mouse peritoneal macrophages were intracellularly stained with 0.25 ug Anti-Mouse CD68 Rabbit Recombinant Antibody (98029-1-RR, Clone: 230504G5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with APC Anti-Mouse CD11b. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of mouse peritoneal macrophages using 98029-1-RR

    FC experiment of mouse peritoneal macrophages using 98029-1-RR

    1x10^6 mouse peritoneal macrophages were intracellularly stained with 0.25 ug Anti-Mouse CD68 Rabbit Recombinant Antibody (98029-1-RR, Clone: 230504G5) (red) or Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98029-1-RR

    Affinity and Kinetic Characterization of 98029-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98029-1-RR against Mouse CD68 were performed. The affinity constant is 10.8 nM.

经过测试的应用

Positive FC (Intra) detected inmouse peritoneal macrophages
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98029-1-RR is CD68 Fusion Protein expressed in E. coli.

Tested Applications FC (Intra) Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD68 antigen
Synonyms Scard1, gp110, CD68 antigen, 230504G5
Calculated Molecular Weight35 kDa
GenBank Accession NumberNM_001291058.1
Gene Symbol Cd68
Gene ID (NCBI) 12514
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP31996-1
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

实验方案

Product Specific Protocols
FC protocol for CD68 antibody 98029-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols