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CoraLite® Plus 647 Anti-Mouse CD68 Rabbit Recombinant Antibody

CD68 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

230504G5

Cat no : CL647-98029

Print datasheet

Synonyms

Scard1, gp110, CD68 antigen, 230504G5

验证数据展示

  • FC experiment of mouse peritoneal macrophages using CL647-98029

    FC experiment of mouse peritoneal macrophages using CL647-98029

    1x10^6 mouse peritoneal macrophages were intracellularly stained with CoraLite® Plus 488 Anti-Mouse CD11b and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD68 Rabbit Recombinant Antibody (CL647-98029, Clone:230504G5), or Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of mouse peritoneal macrophages using CL647-98029

    FC experiment of mouse peritoneal macrophages using CL647-98029

    1x10^6 mouse peritoneal macrophages were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD68 Rabbit Recombinant Antibody (CL647-98029, Clone:230504G5)(red), or Isotype Control(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inmouse peritoneal macrophages
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 98029-1-PBS

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL647-98029 is CD68 Fusion Protein expressed in E. coli.

Tested Applications FC (Intra) Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD68 antigen
Synonyms Scard1, gp110, CD68 antigen, 230504G5
Calculated Molecular Weight35 kDa
GenBank Accession NumberNM_001291058.1
Gene Symbol Cd68
Gene ID (NCBI) 12514
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP31996-1
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD68 antibody CL647-98029Download protocol
Standard Protocols
Click here to view our Standard Protocols