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验证数据展示

  • FC experiment of mouse peritoneal macrophages using PE-98029

    FC experiment of mouse peritoneal macrophages using PE-98029

    1x10^6 mouse peritoneal macrophages were intracellularly stained with 0.1 ug PE Anti-Mouse CD68 Rabbit Recombinant Antibody (PE-98029, Clone: 230504G5) or Isotype Control. Cells were co-stained with APC Anti-Mouse CD11b. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of mouse peritoneal macrophages using PE-98029

    FC experiment of mouse peritoneal macrophages using PE-98029

    1x10^6 mouse peritoneal macrophages were intracellularly stained with 0.1 ug PE Anti-Mouse CD68 Rabbit Recombinant Antibody (PE-98029, Clone: 230504G5) (red) or Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

PE Anti-Mouse CD68 Rabbit Recombinant Antibody

CD68 Recombinant Antibody for FC (Intra)
Cat No. PE-98029

产品说明书

CloneNo. 230504G5

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC (Intra)

别名

Scard1, gp110, CD68 antigen, 230504G5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  PE
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inmouse peritoneal macrophages
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-98029 targets CD68 in FC (Intra) applications and shows reactivity with mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 mouse
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD68 antigen
别名 Scard1, gp110, CD68 antigen, 230504G5
计算分子量35 kDa
GenBank蛋白编号NM_001291058.1
基因名称 Cd68
Gene ID (NCBI) 12514
RRIDAB_3674015
偶联类型 PE Fluorescent Dye
最大激发/发射波长496 nm, 565 nm / 578 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP31996-1
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

实验方案

Product Specific Protocols
FC protocol for PE CD68 antibody PE-98029Download protocol
Standard Protocols
Click here to view our Standard Protocols