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Anti-Mouse CD80/B7-1 Rabbit Recombinant Antibody, PBS Only

CD80/B7-1 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

241371E12

Cat no : 98158-1-PBS

Print datasheet

Synonyms

Cd80, CD80 antigen, Cd28l, B7.1, B7 1

验证数据展示

  • FC experiment of mouse peritoneal macrophages using 98158-1-RR (same clone as 98158-1-PBS)

    FC experiment of mouse peritoneal macrophages using 98158-1-RR (same clone as 98158-1-PBS)

    1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD80 Rabbit Recombinant Antibody (98158-1-RR, Clone: 241371E12) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98158-1-PBS in a different storage buffer formulation.

  • FC experiment of mouse peritoneal macrophages using 98158-1-RR (same clone as 98158-1-PBS)

    FC experiment of mouse peritoneal macrophages using 98158-1-RR (same clone as 98158-1-PBS)

    1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD80/B7-1 Rabbit Recombinant Antibody (98158-1-RR, Clone: 241371E12) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98158-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98158-1-RR

    Affinity and Kinetic Characterization of 98158-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98158-1-RR against Mouse CD80/B7-1 were performed. The affinity constant is 1.05 nM.

产品信息

98158-1-PBS targets CD80/B7-1 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD80 antigen
Synonyms Cd80, CD80 antigen, Cd28l, B7.1, B7 1
Calculated Molecular Weight35 kDa
GenBank Accession NumberNM_009855.2
Gene Symbol Cd80
Gene ID (NCBI) 12519
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ00609-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)