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验证数据展示

  • FC experiment of Human Monocytes-derived dendritic cells using APC-65171

    FC experiment of Human Monocytes-derived dendritic cells using APC-65171

    1X10^6 Human monocytes-derived dendritic cells (induced with GM-CSF+IL-4+TNF-α) were surface stained with 10 ul APC Anti-Human CD83 (APC-65171, Clone:HB15e) (blue) or APC mouse IgG1 isotype control (black). Cells were not fixed.

APC Anti-Human CD83 (HB15e)

CD83 Monoclonal Antibody for FC
Cat No. APC-65171

Print datasheet

CloneNo. HB15e

Host / Isotype

Mouse / Mouse IgG1 kappa

Reactivity

Human, Non-human primates

Applications

FC

Synonyms

B cell activation protein, BL11, CD83, CD83 antigen, CD83 molecule, Cell surface protein HB15, HB15, hCD83

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  APC
Size: 

-/ -

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经过测试的应用

Positive FC detected inHuman monocytes-derived dendritic cells

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

APC-65171 targets CD83 in FC applications and shows reactivity with Human, Non-human primates samples.

Tested Applications FC Application Description
Tested Reactivity Human, Non-human primates
Immunogen Human CD83-transfected Cos cells 种属同源性预测
Host / Isotype Mouse / Mouse IgG1 kappa
Class Monoclonal
Type Antibody
Full Name CD83 molecule
Synonyms B cell activation protein, BL11, CD83, CD83 antigen, CD83 molecule, Cell surface protein HB15, HB15, hCD83
Calculated Molecular Weight 205 aa, 23 kDa
GenBank Accession NumberBC030830
Gene Symbol CD83
Gene ID (NCBI) 9308
ENSEMBL Gene IDENSG00000112149
RRIDAB_2918931
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
UNIPROT IDQ01151
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

实验方案

Product Specific Protocols
FC protocol for APC CD83 antibody APC-65171Download protocol
Standard Protocols
Click here to view our Standard Protocols