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验证数据展示

  • FC experiment of Human Monocytes-derived dendritic cells using APC-65171

    FC experiment of Human Monocytes-derived dendritic cells using APC-65171

    1X10^6 Human monocytes-derived dendritic cells (induced with GM-CSF+IL-4+TNF-α) were surface stained with 10 ul APC Anti-Human CD83 (APC-65171, Clone:HB15e) (blue) or APC mouse IgG1 isotype control (black). Cells were not fixed.

APC Anti-Human CD83 (HB15e)

CD83 Monoclonal Antibody for FC
Cat No. APC-65171

产品说明书

CloneNo. HB15e

宿主/亚型

Mouse / Mouse IgG1 kappa

种属反应性

Human, Non-human primates

应用

FC

别名

B cell activation protein, BL11, CD83, CD83 antigen, CD83 molecule, Cell surface protein HB15, HB15, hCD83

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  APC
规格: 

-/ -

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经过测试的应用

Positive FC detected inHuman monocytes-derived dendritic cells

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

APC-65171 targets CD83 in FC applications and shows reactivity with Human, Non-human primates samples.

经测试应用 FC Application Description
经测试反应性 Human, Non-human primates
免疫原 Human CD83-transfected Cos cells 种属同源性预测
宿主/亚型 Mouse / Mouse IgG1 kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD83 molecule
别名 B cell activation protein, BL11, CD83, CD83 antigen, CD83 molecule, Cell surface protein HB15, HB15, hCD83
计算分子量 205 aa, 23 kDa
GenBank蛋白编号BC030830
基因名称 CD83
Gene ID (NCBI) 9308
ENSEMBL Gene IDENSG00000112149
RRIDAB_2918931
偶联类型 APC Fluorescent Dye
最大激发/发射波长650 nm / 660 nm
形式 Liquid
纯化方式The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
UNIPROT IDQ01151
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

实验方案

Product Specific Protocols
FC protocol for APC CD83 antibody APC-65171Download protocol
Standard Protocols
Click here to view our Standard Protocols