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Anti-Human CD83 (HB15e)

CD83 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human, non-human primates

Applications

FC

Conjugate

Unconjugated

CloneNo.

HB15e

Cat no : 65171-1-Ig

Print datasheet

Synonyms

Cell surface protein HB15, CD83 molecule, CD83 antigen, BL11, B-cell activation protein

验证数据展示

  • FC experiment of Human Monocytes-derived dendritic cells using 65171-1-Ig

    FC experiment of Human Monocytes-derived dendritic cells using 65171-1-Ig

    1X10^6 Human monocytes-derived dendritic cells (induced with GM-CSF+IL-4+TNF-α) were surface stained with 0.5 ug Anti-Human CD83 (65171-1-Ig, Clone:HB15e) and FITC anti-Mouse IgG1 Antibody at dilution 1:50 (green), or stained with 0.5 ug mouse IgG1 isotype control and FITC anti-Mouse IgG1 Antibody at dilution 1:50 (black). Cells were not fixed.

经过测试的应用

Positive FC detected inHuman monocytes-derived dendritic cells

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65171-1-Ig targets CD83 in FC applications and shows reactivity with human, non-human primates samples.

Tested Applications FC Application Description
Tested Reactivity human, non-human primates
Immunogen Human CD83-transfected Cos cells 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD83 molecule
Synonyms Cell surface protein HB15, CD83 molecule, CD83 antigen, BL11, B-cell activation protein
Calculated Molecular Weight 205 aa, 23 kDa
GenBank Accession NumberBC030830
Gene Symbol CD83
Gene ID (NCBI) 9308
ENSEMBL Gene IDENSG00000112149
RRIDAB_2918447
Conjugate Unconjugated
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
UNIPROT IDQ01151
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).

实验方案

Product Specific Protocols
FC protocol for CD83 antibody 65171-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols