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验证数据展示

  • FC experiment of human PBMCs using 98108-1-RR (same clone as 98108-1-PBS)

    FC experiment of human PBMCs using 98108-1-RR (same clone as 98108-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD84 Rabbit Recombinant Antibody (98108-1-RR, Clone: 241345A3) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD4 (OKT4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98108-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98108-1-RR (same clone as 98108-1-PBS)

    FC experiment of human PBMCs using 98108-1-RR (same clone as 98108-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD84 Rabbit Recombinant Antibody (98108-1-RR, Clone: 241345A3) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98108-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01112-2

    Sandwich ELISA standard curve of MP01112-2

    Sandwich ELISA standard curve of MP01112-2, Human CD84 Recombinant Matched Antibody Pair - PBS only. 84245-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02347. 98108-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • Sample test of MP01112-2

    Sample test of MP01112-2

    The mean CD84 concentration was determined to be 59.65 ng/mL in Raji cell extract based on a 1.0 mg/mL extract load and 79.95 ng/mL in Daudi cell extract based on a 1.8 mg/mL extract load.

  • Sample test of MP01112-2

    Sample test of MP01112-2

    Serum of six individual healthy human donors was measured. The human CD84 concentration of detected samples was determined to be 13,292.9 pg/mL with a range of 8,538.9 - 16,254.6 pg/mL.

  • Cytometric bead array standard curve of MP01112-1

    Cytometric bead array standard curve of MP01112-1

    Cytometric bead array standard curve of MP01112-1, CD84 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98108-1-PBS. Detection antibody: 84245-2-PBS. Standard: RP02347. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01112-2

    Cytometric bead array standard curve of MP01112-2

    Cytometric bead array standard curve of MP01112-2, CD84 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84245-1-PBS. Detection antibody: 98108-1-PBS. Standard: RP02347. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 98108-1-RR

    Affinity and Kinetic Characterization of 98108-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98108-1-RR against Human CD84 were performed. The affinity constant is 1.14 nM.

Anti-Human CD84 Rabbit Recombinant Antibody, PBS Only

CD84 Uni-rAbTM Recombinant Antibody for FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 98108-1-PBS

产品说明书

CloneNo. 241345A3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Hly9-beta, Hly9 beta, hCD84, Cell surface antigen MAX.3, CD84 molecule

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

98108-1-PBS targets CD84 as part of a matched antibody pair:

MP01112-1: 98108-1-PBS capture and 84245-2-PBS detection (validated in Cytometric bead array)

MP01112-2: 84245-1-PBS capture and 98108-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD84 molecule
别名 Hly9-beta, Hly9 beta, hCD84, Cell surface antigen MAX.3, CD84 molecule
计算分子量 345 aa, 39 kDa
GenBank蛋白编号BC020063
基因名称 CD84
Gene ID (NCBI) 8832
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UIB8
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD84, also known as SLAMF5, is a member of the CD2 subfamily of the immunoglobulin receptor superfamily (PMID: 9310491). CD84 is a single-chain type-I glycoprotein composed of two extracellular Ig-like domains, a hydrophobic transmembrane region, and a cytoplasmic domain. It is broadly expressed on almost all leukocyte subsets. CD84 functions as a homophilic adhesion molecule, whose signaling can activate or inhibit leukocyte function depending on the cell type and its stage of activation or differentiation (PMID: 30522694).