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Anti-Human CD85j / LILRB1 (GHI/75)

CD85j / LILRB1 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

GHI/75

Cat no : 65238-1-Ig

Print datasheet

Synonyms

LILRB1, ILT2, ILT 2, FLJ37515, CD85j

验证数据展示

  • FC experiment of human PBMCs using 65238-1-Ig

    FC experiment of human PBMCs using 65238-1-Ig

    1X10^6 PBMC were surface stained with 0.2 ug Anti-Human LILRB1 (65238-1-Ig, Clone: GHI/75) and APC-Donkey anti-Mouse IgG at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Monocytes are gated. Cells were not fixed.

  • FC experiment of human PBMCs using 65238-1-Ig

    FC experiment of human PBMCs using 65238-1-Ig

    1X10^6 PBMC were surface stained with 0.2 ug Anti-Human LILRB1 (65238-1-Ig, Clone: GHI/75) and APC-Donkey anti-Mouse IgG at dilution 1:1000, or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10). Monocytes are gated. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite® Plus 555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65238-1-Ig targets CD85j / LILRB1 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Human Hairy Cell Leukemia Spleen Cells 种属同源性预测
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1
Synonyms LILRB1, ILT2, ILT 2, FLJ37515, CD85j
Calculated Molecular Weight 71 kDa
GenBank Accession NumberBC015731
Gene Symbol LILRB1
Gene ID (NCBI) 10859
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ8NHL6
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

LILRB1, also named as CD85j or ILT2, includes four C2 Ig domains binding HLA class I molecules in the extracellular region and a cytoplasmic domain containing four immunoreceptor tyrosine-based inhibition motifs (ITIM). LILRB1 is a surface glycoprotein detected on the surface of B cells, monocytes, dendritic cells, T cells and NK cells.

实验方案

Product Specific Protocols
FC protocol for CD85j / LILRB1 antibody 65238-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols