验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
Planning an IHC experiment? We recommend our IHCeasy LILRB1 Ready-To-Use IHC Kit. LILRB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65238-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-65238 targets CD85j / LILRB1 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 | Human Hairy Cell Leukemia Spleen Cells 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1 |
| 别名 | LILRB1, ILT2, ILT 2, FLJ37515, CD85j |
| 计算分子量 | 71 kDa |
| GenBank蛋白编号 | BC015731 |
| 基因名称 | LILRB1 |
| Gene ID (NCBI) | 10859 |
| RRID | AB_2935022 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | Q8NHL6 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
LILRB1, also named as CD85j or ILT2, includes four C2 Ig domains binding HLA class I molecules in the extracellular region and a cytoplasmic domain containing four immunoreceptor tyrosine-based inhibition motifs (ITIM). LILRB1 is a surface glycoprotein detected on the surface of B cells, monocytes, dendritic cells, T cells and NK cells.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 CD85j / LILRB1 antibody CL647-65238 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |