• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 647 Anti-Human CD85j / LILRB1 (GHI/75)

CD85j / LILRB1 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

GHI/75

Cat no : CL647-65238

Print datasheet

Synonyms

LILRB1, ILT2, ILT 2, FLJ37515, CD85j

验证数据展示

  • FC experiment of human PBMCs using CL647-65238

    FC experiment of human PBMCs using CL647-65238

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD85j / LILRB1 (CL647-65238, Clone:GHI/75) or Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL647-65238

    FC experiment of human PBMCs using CL647-65238

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD85j / LILRB1 (CL647-65238, Clone:GHI/75) or Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65238-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65238 targets CD85j / LILRB1 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Human Hairy Cell Leukemia Spleen Cells 种属同源性预测
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1
Synonyms LILRB1, ILT2, ILT 2, FLJ37515, CD85j
Calculated Molecular Weight 71 kDa
GenBank Accession NumberBC015731
Gene Symbol LILRB1
Gene ID (NCBI) 10859
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ8NHL6
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

LILRB1, also named as CD85j or ILT2, includes four C2 Ig domains binding HLA class I molecules in the extracellular region and a cytoplasmic domain containing four immunoreceptor tyrosine-based inhibition motifs (ITIM). LILRB1 is a surface glycoprotein detected on the surface of B cells, monocytes, dendritic cells, T cells and NK cells.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD85j / LILRB1 antibody CL647-65238Download protocol
Standard Protocols
Click here to view our Standard Protocols